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Diagnostic Testing for PEDV Antigen and Antibody Detection

Since PEDV entered the United States, millions of piglets have died. Veterinary diagnostic laboratories quickly developed sensitive and specific real-time, reverse-transcription polymerase chain reaction (RRT-PCR) assays to detect PEDV in a variety of porcine and environmental samples. However, lack of a reliable method to evaluate immunity has hindered progress in PEDV research, control and prevention. Key items needed to be addressed. These included the development and validation of enzyme-linked immunosorbent assay (ELISA) for serological and environmental testing to determine the history of exposure, efficacy of feedback and duration of immunity. This project was implemented to produce PEDV viral protein antigens, produce an ELISA assay and validate it for routine diagnostic analysis. [Source: Pork Checkoff Research Projects]

Key Points:

  • The study developed and validated a test (ELISA assay) to predict previous Porcine Epidemic Diarrhea Virus (PEDV) exposure.
  • The test provides high PEDV sensitivity and specificity, allowing clinical diagnostic laboratories to use one test.
  • This application will help advance PEDV prevention and control strategies.

Source: AASV


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Navigating Challenges On A Sheep Farm!

Video: Navigating Challenges On A Sheep Farm!

In today's episode of our daily sheep farming vlog at Ewetopia Farms, we find ourselves faced with a multitude of tasks and limited time to complete them all. However, we are determined to make progress! Our main focus today was to train the Dorset triplets to drink from bottles without the need for us to hold them. Arnie even took on the challenge of feeding bottles to the Suffolk lambs, giving him a taste of what it's like to be mobbed by hungry little ones!

This year, our lambs are growing at an impressive rate, which brings both joy and unexpected challenges. Some of the lambs have been getting stuck under the feeders, while others struggle to enter or exit the creep pen. To address the feeder issue, we diligently cleaned out the bedding pack surrounding the feeders, providing the lambs with more space. As for the lambs' difficulty in accessing the creep pen, we plan to separate the larger lambs and relocate them to another pen soon.

With a large number of sheep on the farm, ensuring everyone is fed each day leaves us with limited extra time to tackle additional tasks. Nevertheless, we believe in taking small steps towards our goals. Join us in this episode as we navigate through the challenges and work towards the well-being of our flock