Inclusion of dried distillers grains with solubles (DDGS) in swine diets above 20% can negatively impact growth performance of pigs (Whitney et al., 2006; Linneen et al., 2008). An imbalance in the branched chain amino acids (BCAA), specifically excess leucine, is one potential explanation of this decrease in performance. In 2019, Cemin and coworkers conducted a meta-analysis to derive a performance prediction model for BCAA levels in the diet. Recently, Kerkaert and coworkers (2021) conducted a study to validate this prediction model. Results from their study indicated that the negative effects of excess dietary leucine can be mitigated with high inclusion of valine (Val) in the grower period and high inclusion of isoleucine (Ile) in the finishing period. Therefore, two studies were conducted to validate the Val and Ile requirement from the prediction model in diets containing DDGS during the growing and finishing periods.The studies were conducted at a commercial research facility in southwestern Minnesota to determine the standardized ileal digestible (SID) Val:Lysine (Lys) requirement of the growing pig fed diets containing 30% DDGS. A total of 2,425 pigs with an initial body weight (BW) of 87 pounds were used in a 28-day growing study. One of six dietary treatments were randomly assigned to pens containing 27 pigs with approximately equal number of barrows and gilts. Dietary treatments consisted of diets containing 30% DDGS with SID Val:Lys ratios of 60, 65, 70, 75 or 80% or a corn-soybean meal (SBM) diet. The addition of L-Valine was used to create the titrated levels of Val:Lys. Lysine was formulated at 95% of the pig’s requirement, and the diets contained similar dietary energy. Diets containing DDGS provided an Ile:Lys ratio of 60% and a Tryptophan (Trp) to Lys ratio of 21%. Results from this study indicated that the SID Val:Lys ratio of 68% would provide maximum growth performance when pigs are fed 30% DDGS in the growing period. The Cemin and coworkers (2019) prediction model accurately described the trend SID Val had on pig performance when pigs were fed DDGS diets. However, pigs fed a corn-SBM diet still outperformed pigs fed diets containing DDGS (Figure 1).Figure 1. Growing pig valine study performance results.