Because ASFV is a highly contagious virus, and countries such as those in the Americas, Australia and New Zealand where the disease is still absent, research with African swine fever virus (ASFV) can only be conducted in a highly restricted biosecurity level 3 facility. Consequently, this has resulted in only a few laboratories in the world that have regulatory approval to work with this virus. These biosecurity restrictions also limit the capability of evaluating ASFV survival and inactivation in various feed ingredients under real world feed supply chain demonstrations because unlike many RNA viruses, no suitable surrogate has been available for ASFV.
African swine fever virus is a member of the Asfarviridae family which is part of a larger group of virus families that are classified as nucleocytoplasmic large DNA viruses (NCLDVs) and evolved from a common ancestor. These NCLDVs are found in a variety of environments, and can infect humans (Poxviridae), fish (Iridoviridae), insects (Ascoviridae), swine (Asfarviridae), amoeba (Marseilleviridae and Mimiviridae) and algae (Phycodnaviridae).
Until now, no surrogate NCLDV with similar features to that of ASFV, nor any other virus with suitable surrogate properties, have been proposed for use in studies to evaluate ASFV survival and inactivation in feed ingredients and complete feeds. Emiliania huxleyi virus strain 86 (EhV-86) is an ecologically important NCLDV which controls blooms of the marine unicellular phytoplankton Emiliania huxleyi and shares many important features with ASFV. Both ASFV and EhV-86 share many physical characteristics, such as complex virion ultrastructure and sensitivity profile to time and temperature exposure. In fact, EhV-86 has recently been shown to be one of the most thermally stable viruses known, with temperatures up to 100°C damaging most of the virus particles yet leaving a subset of intact and potentially viable particles for future re-infections. Given the similarities shared between ASFV and EhV-86, proposed the use of EhV-86 as a surrogate for ASFV.
ASFV is stable in a variety of environments, including animal feed ingredients as shown in previous laboratory experiments and simulations.
Emiliania huxleyi virus has a restricted host range limited to a species of marine algae called Emiliania huxleyi. This algal NCLDV has many similar morphological and physical characteristics to ASFV thereby making it a safe surrogate, with results that are applicable to ASFV and suitable for use in real-world experiments.
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