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New Tool Enables Faster, More Cost-Effective Genome Editing of Traits to Improve Agriculture Sustainability

With the goal of reducing the time and cost it takes to bring an improved crop to the marketplace to improve agriculture sustainability, research conducted in the laboratory of Keith Slotkin, Ph.D., and his colleagues in the Plant Transformation Facility at the Donald Danforth Plant Science Center, was recently published in the journal Nature. The publication "Transposase-assisted target site integration for efficient plant genome engineering" focuses on technology called TATSI (Transposase-Assisted Target Site Integration), which uses transposable elements to integrate custom DNA into specific sites in plant genomes.

The TATSI technology takes advantage of over two billion years of evolution of plant , which naturally function as honed  to insert DNA into the . The  and high-precision target site integration of custom DNA into plant genomes enables the faster and less expensive production of gene-edited plants to address global challenges in agriculture, climate and the environment.

A critical bottleneck in modern crop improvement is the low frequency and error-prone integration of foreign DNA into the plant genome, hampering genome editing approaches for crop improvement. The CRISPR/Cas system functions like a pair of molecular "scissors" to cut the genome and introduce site-specific changes to the DNA. But current methods lack robust ways to add custom DNA accurately and efficiently at those edited sites.

TATSI technology takes advantage of the molecular "glue" feature of transposable elements to provide custom "cut-and-paste" genome editing when combined with CRISPR/Cas. The "scissors + glue" combination enables an order-of-magnitude increase in the rate of targeted DNA integration in , allowing for custom improvement of plants through the addition of important traits such as virus resistance, elevated nutrient levels, or better oil composition.

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Why Your Food Future Could be Trapped in a Seed Morgue

Video: Why Your Food Future Could be Trapped in a Seed Morgue

In a world of PowerPoint overload, Rex Bernardo stands out. No bullet points. No charts. No jargon. Just stories and photographs. At this year’s National Association for Plant Breeding conference on the Big Island of Hawaii, he stood before a room of peers — all experts in the science of seeds — and did something radical: he showed them images. He told them stories. And he asked them to remember not what they saw, but how they felt.

Bernardo, recipient of the 2025 Lifetime Achievement Award, has spent his career searching for the genetic treasures tucked inside what plant breeders call exotic germplasm — ancient, often wild genetic lines that hold secrets to resilience, taste, and traits we've forgotten to value.

But Bernardo didn’t always think this way.

“I worked in private industry for nearly a decade,” he recalls. “I remember one breeder saying, ‘We’re making new hybrids, but they’re basically the same genetics.’ That stuck with me. Where is the new diversity going to come from?”

For Bernardo, part of the answer lies in the world’s gene banks — vast vaults of seed samples collected from every corner of the globe. Yet, he says, many of these vaults have quietly become “seed morgues.” “Something goes in, but it never comes out,” he explains. “We need to start treating these collections like living investments, not museums of dead potential.”

That potential — and the barriers to unlocking it — are deeply personal for Bernardo. He’s wrestled with international policies that prevent access to valuable lines (like North Korean corn) and with the slow, painstaking science of transferring useful traits from wild relatives into elite lines that farmers can actually grow. Sometimes it works. Sometimes it doesn’t. But he’s convinced that success starts not in the lab, but in the way we communicate.

“The fact sheet model isn’t cutting it anymore,” he says. “We hand out a paper about a new variety and think that’s enough. But stories? Plants you can see and touch? That’s what stays with people.”

Bernardo practices what he preaches. At the University of Minnesota, he helped launch a student-led breeding program that’s working to adapt leafy African vegetables for the Twin Cities’ African diaspora. The goal? Culturally relevant crops that mature in Minnesota’s shorter growing season — and can be regrown year after year.

“That’s real impact,” he says. “Helping people grow food that’s meaningful to them, not just what's commercially viable.”

He’s also brewed plant breeding into something more relatable — literally. Coffee and beer have become unexpected tools in his mission to make science accessible. His undergraduate course on coffee, for instance, connects the dots between genetics, geography, and culture. “Everyone drinks coffee,” he says. “It’s a conversation starter. It’s a gateway into plant science.”