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Beetroot Dye Helps Biologists 'track and trace' Symbiotic Fungi for Sustainable Farming

Beetroot Dye Helps Biologists 'track and trace' Symbiotic Fungi for Sustainable Farming

Almost all crop plants form associations with a particular type of fungi—called arbuscular mycorrhiza fungi—in the soil, which greatly expand their root surface area. This mutually beneficial interaction boosts the plant's ability to take up nutrients that are vital for growth.

The more nutrients plants obtain naturally, the less artificial fertilizers are needed. Understanding this , as the first step towards potentially enhancing it, is an ongoing research challenge. Progress is likely to pay huge dividends for agricultural productivity.

In a study published in the journal PLOS Biology, researchers used the bright red pigments of beetroot—called betalains—to visually track soil fungi as they colonized plant roots in a living plant.

"We can now follow how the relationship between the fungi and plant root develops, in , from the moment they come into contact. We previously had no idea about what happened because there was no way to visualize it in a living plant without the use of elaborate microscopy," said Dr. Sebastian Schornack, a researcher at the University of Cambridge's Sainsbury Laboratory and joint senior author of the paper.

To achieve their results, the researchers engineered two model plant species—a legume and a tobacco plant—so that they would produce the highly visible betalain pigments when arbuscular mycorrhiza fungi were present in their roots. This involved combining the control regions of two genes activated by  with genes that synthesize red-colored betalain pigments.

The plants were then grown in a transparent structure so that the root system was visible, and images of the roots could be taken with a flatbed scanner without disturbing the plants.

Using their technique, the researchers could select red pigmented parts of the root system to observe the fungus more closely as it entered individual plant cells and formed elaborate tree-like structures—called arbuscules—which grow inside the plant's roots. Arbuscules take up nutrients from the soil that would otherwise be beyond the reach of the plant.

Other methods exist to visualize this process, but these involve digging up and killing the plant and the use of chemicals or expensive microscopy. This work makes it possible for the first time to watch by eye and with simple imaging how symbiotic fungi start colonizing living plant roots, and inhabit parts of the plant  over time.

"This is an exciting new tool to visualize this, and other, important plant processes. Beetroot pigments are a distinctive color, so they're very easy to see. They also have the advantage of being natural plant pigments, so they are well tolerated by plants," said Dr. Sam Brockington, a researcher in the University of Cambridge's Department of Plant Sciences, and joint senior author of the paper.

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