Rice Breeding Breakthrough could Feed Billions

Jan 11, 2023

By Andy Fell

Rice breeding

Phenotype, panicle fertility, and grain quality of progeny plants of selected apomictic events harboring the T314 and T315 T-DNA constructs. A Phenotypes of plants grown under controlled greenhouse conditions. Left: Five F2 progeny plants derived from the self-fertilization of BRS-CIRAD 302 compared to a BRS-CIRAD 302 F1 plant. Right: Three T1 progenies from T314 15.1 event compared to a BRS-CIRAD 302 F1 plant. B Phenotypes of T2 progenies grown under controlled greenhouse conditions: 5–6 T2 progeny plants of a T1 plant of events T314 15.1, T314 37.7, T315 5.4, and T315 8.1 are compared to a BRS-CIRAD 302 F1 plant. Senescent leaves of the plants have been removed for photographing. C Panicles of the BRS-CIRAD 302 F1 hybrid and of T314 15.1 T2 plants. The master panicles of five distinct plants have been pooled for photographing. D Distribution of seed filling rate among BRS-CIRAD 302 F1 plants, and T2 progeny plants of T314 events (15.1 and 37.7) and T315 events (5.4 and 8.1 events). Average panicle fertilities of the apomictic lines represent 75.8%, 60.7, 79.9%, and 74.7%, respectively, of that of control plants (100%) (dotted red line). Significance of the differences are based on Duncan's test, dof = 4, confidence interval of 95%. E Husked and dehulled seeds of 1 F and D24 parents, F1 and F2 generations and apomictic lines. Upper: 1 F and D24 parents, F1 hybrid seeds harvested on 1 F parent, F2 seeds harvested on the F1 hybrid. Lower: T3 seeds harvested from apomictic plants in the four selected apomictic lines. F Starch and amylose content of F2 seeds and T3 seeds harvested from BRS-CIRAD 302 F1 plants and T2 apomictic plants, respectively. Different letters indicate significant differences (α-risk = 0.05) in a Kruskal–Wallis test: Event T314 37.7 exhibits a significantly lower starch content than F2 seeds and seeds of event T315 8.1 and 5.4.

An international team has succeeded in propagating a commercial hybrid rice strain as a clone through seeds with 95 percent efficiency.

First-generation hybrids of crop plants often show higher performance than their parent strains, a phenomenon called hybrid vigor. But this does not persist if the hybrids are bred together for a second generation. So when farmers want to use high-performing hybrid plant varieties, they need to purchase new seed each season.

Rice, the staple crop for half the world's population, is relatively costly to breed as a hybrid for a yield improvement of about 10 percent. This means that the benefits of rice hybrids have yet to reach many of the world's farmers, said Gurdev Khush, adjunct professor emeritus in the Department of Plant Sciences at the University of California, Davis. Working at the International Rice Research Institute from 1967 until retiring to UC Davis in 2002, Khush led efforts to create new rice high-yield rice varieties, work for which he received the World Food Prize in 1996.

One solution to this would be to propagate hybrids as clones that would remain identical from generation to generation without further breeding. Many wild plants can produce seeds that are clones of themselves, a process called apomixis.

"Once you have the hybrid, if you can induce apomixis, then you can plant it every year," Khush said.

However, transferring apomixis to a major crop plant has proved difficult to achieve.

One step to cloned hybrid seeds

In 2019, a team led by Professor Venkatesan Sundaresan and Assistant Professor Imtiyaz Khanday at the UC Davis Departments of Plant Biology and Plant Sciences achieved apomixis in rice plants, with about 30 percent of seeds being clones.

Sundaresan, Khanday and colleagues in France, Germany and Ghana have now achieved a clonal efficiency of 95 percent, using a commercial hybrid rice strain, and shown that the process could be sustained for at least three generations.

The single-step process involves modifying three genes called MiMe which cause the plant to switch from meioisis, the process that plants use to form egg cells, to mitosis, in which a cell divides into two copies of itself. Another gene modification induces apomixis. The result is a seed that can grow into a plant genetically identical to its parent.

The method would allow seed companies to produce hybrid seeds more rapidly and at larger scale, as well as providing seed that farmers could save and replant from season to season, Khush said.

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Video: The FCDC and AgSmart Bring Plant Breeding to a Wider Audience

In the vast prairies of Alberta, Olds College’s Field Crop Development Centre (FCDC) stands as a beacon of innovation and research in the agricultural world. The institution has become a key player in advancing agricultural technologies and practices. The FCDC’s commitment to applied research has driven them to seek effective means of disseminating their findings and creating a positive impact on the farming community.

One such avenue that aligns with their mission is AgSmart, an event dedicated to showcasing cutting-edge agricultural technologies. The coming together of the FCDC’s annual Field Day and Ag Smart has proved to be a natural fit, fostering a synergy that benefits both parties and propels the agriculture industry forward. The FCDC Field Day took part in conjunction with AgSmart for the first time this week on Aug. 1-2 in Olds, Alta. FCDC Program Director Kofi Agblor and Olds College VP of Development Todd Ormann sat down for an interview with Marc Zienkiewicz to discuss the significance of the two events taking part together and what the future holds.

The Intersection of Research and Technology The essence of the FCDC lies in its dedication to plant breeding and new seed varieties, particularly barley and triticale. While conducting research is essential, it becomes meaningful when its benefits are shared with the wider community. This is where AgSmart steps in, providing a key venue for the FCDC to showcase their research. This union between research and technology creates a holistic and enriching experience for farmers, ranchers, and industry professionals, the pair said.

Seeds as Technology For the FCDC, the partnership with AgSmart goes beyond mere event collaboration. It is about creating an environment that bridges the gap between seeds and smart technology, Ormann said. The college believes that for technology to truly revolutionize agriculture, it must begin with a strong foundation — high-quality seeds. As the saying goes, “it all starts with a seed.” To demonstrate this critical aspect, the collaboration aims to showcase the seed value chain as an integral part of the smartphone.

The Birth of a Powerful Alliance The idea of joining forces emerged when staff realized the potential synergy between AgSmart and the FCDC Field Day. With just a few days separating the two events, a proposal was put forward to merge them. The marketing and communications teams from both sides worked seamlessly to ensure the essence of both events remained intact, creating a powerful alliance that leverages the strengths of each, Agblor said.

Driving Advancements in Breeding For Agblor, the partnership with AgSmart has tremendous potential to drive advancements in breeding and other technology. With technologies like drones and imaging becoming integral to phenotyping, breeding is no longer confined to vast fields to assess thousands of plants manually. Instead, it benefits from the data-rich insights brought about by smart technologies. These advancements make breeding more efficient, precise, and instrumental in shaping the future of agriculture.

Overcoming Challenges Together While the partnership between Olds College and Ag Smart has been a resounding success, there are challenges on the horizon. Securing stable funding for long-term breeding initiatives is crucial to sustain progress. The college is committed to navigating these challenges and investing in agriculture’s future sustainably, Agblor said.