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Novel P22-Monoclonal Antibody Based Blocking ELISA For The Detection Of African Swine Fever Virus Antibodies In Serum

African swine fever (ASF) is a highly infectious and fatal disease of pigs. Due to the complexity of the ASF virus (ASFV) and various clinical forms of the disease, a wide range of highly effective and robust sero-diagnostic assays are required.

The use of the most antigenic ASFV proteins is highly crucial to improving sero-diagnostic assays. Currently, only a few highly antigenic recombinant proteins have been tested and validated for use as reagents in ASF sero-diagnostic assays. So far, three ELISA kits based on the recombinant proteins P72, P30, and PP62 have been approved.

In this study, based on the recombinant P22 protein, a highly sensitive, specific, and rapid P22 monoclonal antibody-based blocking ELISA (mAb-bELISA) assay was developed to detect serum antibodies induced by genotype I and II ASFVs to detect ASFV antibodies. A total of 806 pig serum samples were tested to evaluate the performance of the diagnostic assay. The assay was able to detect ASFV antibodies as early as 9 days post-infection.

Based on this study, the noval P22-mAb based bELISA assay can be used for rapid and accurate detection of antibodies against ASFV, which will play a valuable role in the containment and prevention of ASF as an alternative to other serological diagnostic methods. Also, this study will assist researchers to further investigate the immunogenic importance of the P22 protein in ASFV infection.

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Carrying on a Pig Farming Legacy with Purpose and Progress

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After losing his father in a car accident, James Lamb took on the responsibility of the family farm. What started as a way to honor his dad’s legacy has grown into something more. Now, a contract grower for Prestage Farms, a family-owned and operated pork and poultry producer in North Carolina, he runs his own nursery pig operation with sustainability top of mind. From animal welfare to clean, well-managed spaces and smarter manure management strategies, he’s carrying on his family’s legacy while raising the bar for responsible and sustainable pork production.